Review



sapiens mcherry gate16 gabarapl2  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Addgene inc sapiens mcherry gate16 gabarapl2
    Figure 5. Recruitment of autophagy machinery, including LC3B homologs, to stalled AVs is not sufficient to rescue the biogenesis defect. (A–E) Representative maximal projection micrographs of the distal neurites of fixed DRG neurons from aged mice. Stalled AVs are identified by colocalization of anti-ATG5 (green) and anti-ATG9 (blue). Antibodies to other autophagy components are visualized in red: ATG13 (A) or elongation complex components ATG12 (B), ATG7 (C), ATG16L1 (D), and ATG3 (E). Arrowheads denote colocalization state of AVs (stalled, filled arrowheads). Borders of magnifications of indicated puncta denote channel or colocalization state in merge. (F–I) Time series (channels merged) of Halo-ATG5, mAtg8s (mCh-GABARAP in F, <t>mCh-GABARAPL2/GATE16</t> in G, Figure 5 continued on next page
    Sapiens Mcherry Gate16 Gabarapl2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sapiens mcherry gate16 gabarapl2/product/Addgene inc
    Average 90 stars, based on 4 article reviews
    sapiens mcherry gate16 gabarapl2 - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "Expression of WIPI2B counteracts age-related decline in autophagosome biogenesis in neurons"

    Article Title: Expression of WIPI2B counteracts age-related decline in autophagosome biogenesis in neurons

    Journal: eLife

    doi: 10.7554/elife.44219

    Figure 5. Recruitment of autophagy machinery, including LC3B homologs, to stalled AVs is not sufficient to rescue the biogenesis defect. (A–E) Representative maximal projection micrographs of the distal neurites of fixed DRG neurons from aged mice. Stalled AVs are identified by colocalization of anti-ATG5 (green) and anti-ATG9 (blue). Antibodies to other autophagy components are visualized in red: ATG13 (A) or elongation complex components ATG12 (B), ATG7 (C), ATG16L1 (D), and ATG3 (E). Arrowheads denote colocalization state of AVs (stalled, filled arrowheads). Borders of magnifications of indicated puncta denote channel or colocalization state in merge. (F–I) Time series (channels merged) of Halo-ATG5, mAtg8s (mCh-GABARAP in F, mCh-GABARAPL2/GATE16 in G, Figure 5 continued on next page
    Figure Legend Snippet: Figure 5. Recruitment of autophagy machinery, including LC3B homologs, to stalled AVs is not sufficient to rescue the biogenesis defect. (A–E) Representative maximal projection micrographs of the distal neurites of fixed DRG neurons from aged mice. Stalled AVs are identified by colocalization of anti-ATG5 (green) and anti-ATG9 (blue). Antibodies to other autophagy components are visualized in red: ATG13 (A) or elongation complex components ATG12 (B), ATG7 (C), ATG16L1 (D), and ATG3 (E). Arrowheads denote colocalization state of AVs (stalled, filled arrowheads). Borders of magnifications of indicated puncta denote channel or colocalization state in merge. (F–I) Time series (channels merged) of Halo-ATG5, mAtg8s (mCh-GABARAP in F, mCh-GABARAPL2/GATE16 in G, Figure 5 continued on next page

    Techniques Used:



    Similar Products

    sapiens mcherry gate16 gabarapl2  (Addgene inc)
    90
    Addgene inc sapiens mcherry gate16 gabarapl2
    Figure 5. Recruitment of autophagy machinery, including LC3B homologs, to stalled AVs is not sufficient to rescue the biogenesis defect. (A–E) Representative maximal projection micrographs of the distal neurites of fixed DRG neurons from aged mice. Stalled AVs are identified by colocalization of anti-ATG5 (green) and anti-ATG9 (blue). Antibodies to other autophagy components are visualized in red: ATG13 (A) or elongation complex components ATG12 (B), ATG7 (C), ATG16L1 (D), and ATG3 (E). Arrowheads denote colocalization state of AVs (stalled, filled arrowheads). Borders of magnifications of indicated puncta denote channel or colocalization state in merge. (F–I) Time series (channels merged) of Halo-ATG5, mAtg8s (mCh-GABARAP in F, <t>mCh-GABARAPL2/GATE16</t> in G, Figure 5 continued on next page
    Sapiens Mcherry Gate16 Gabarapl2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sapiens mcherry gate16 gabarapl2/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    sapiens mcherry gate16 gabarapl2 - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier
    mcherry gate16  (Addgene inc)
    90
    Addgene inc mcherry gate16
    Figure 5. Recruitment of autophagy machinery, including LC3B homologs, to stalled AVs is not sufficient to rescue the biogenesis defect. (A–E) Representative maximal projection micrographs of the distal neurites of fixed DRG neurons from aged mice. Stalled AVs are identified by colocalization of anti-ATG5 (green) and anti-ATG9 (blue). Antibodies to other autophagy components are visualized in red: ATG13 (A) or elongation complex components ATG12 (B), ATG7 (C), ATG16L1 (D), and ATG3 (E). Arrowheads denote colocalization state of AVs (stalled, filled arrowheads). Borders of magnifications of indicated puncta denote channel or colocalization state in merge. (F–I) Time series (channels merged) of Halo-ATG5, mAtg8s (mCh-GABARAP in F, <t>mCh-GABARAPL2/GATE16</t> in G, Figure 5 continued on next page
    Mcherry Gate16, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mcherry gate16/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    mcherry gate16 - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 5. Recruitment of autophagy machinery, including LC3B homologs, to stalled AVs is not sufficient to rescue the biogenesis defect. (A–E) Representative maximal projection micrographs of the distal neurites of fixed DRG neurons from aged mice. Stalled AVs are identified by colocalization of anti-ATG5 (green) and anti-ATG9 (blue). Antibodies to other autophagy components are visualized in red: ATG13 (A) or elongation complex components ATG12 (B), ATG7 (C), ATG16L1 (D), and ATG3 (E). Arrowheads denote colocalization state of AVs (stalled, filled arrowheads). Borders of magnifications of indicated puncta denote channel or colocalization state in merge. (F–I) Time series (channels merged) of Halo-ATG5, mAtg8s (mCh-GABARAP in F, mCh-GABARAPL2/GATE16 in G, Figure 5 continued on next page

    Journal: eLife

    Article Title: Expression of WIPI2B counteracts age-related decline in autophagosome biogenesis in neurons

    doi: 10.7554/elife.44219

    Figure Lengend Snippet: Figure 5. Recruitment of autophagy machinery, including LC3B homologs, to stalled AVs is not sufficient to rescue the biogenesis defect. (A–E) Representative maximal projection micrographs of the distal neurites of fixed DRG neurons from aged mice. Stalled AVs are identified by colocalization of anti-ATG5 (green) and anti-ATG9 (blue). Antibodies to other autophagy components are visualized in red: ATG13 (A) or elongation complex components ATG12 (B), ATG7 (C), ATG16L1 (D), and ATG3 (E). Arrowheads denote colocalization state of AVs (stalled, filled arrowheads). Borders of magnifications of indicated puncta denote channel or colocalization state in merge. (F–I) Time series (channels merged) of Halo-ATG5, mAtg8s (mCh-GABARAP in F, mCh-GABARAPL2/GATE16 in G, Figure 5 continued on next page

    Article Snippet: Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Genetic reagent (Mus musculus) GFP-LC3B RIKEN BioResource Center in Japan; PMID: 14699058 RRID:IMSR_RBRC00806 Genetic reagent (M. musculus) C57BL/6J Jackson Laboratory Cat # 000664; RRID:IMSR_JAX:000664 Cell line (Homo sapiens) HEK293 Gibco (ThermoFisher) Cat # R70507 Authenticated by STR profiling; tested negative for mycoplasma Cell line (H. sapiens) HeLa-M A. Peden (Cambridge Institute for Medical Research) Authenticated by STR profiling; tested negative for mycoplasma Transfected construct (H. sapiens) mCherry-ATG13 This paper Subcloned from RRID:Addgene_22875 Transfected construct (M. musculus) mCherry-ATG5 Addgene; PMID:16645637 RRID:Addgene_13095 Transfected construct (H. sapiens) Halo-ATG5 This paper Subcloned from RRID:Addgene_13095 Transfected construct (H. sapiens) SNAP-ATG9 This paper Subcloned from RRID:Addgene_60609 Transfected construct (H. sapiens) Halo-ATG9 This paper Subcloned from RRID:Addgene_60609 Transfected construct (H. sapiens) SNAP-WIPI1A This paper Subcloned from RRID:Addgene_38272 Transfected construct (M. musculus) Halo-DFCP1 This paper Subcloned from RRID:Addgene_38269 Transfected construct (H. sapiens) mScarlet-LC3A This paper Subcloned from RRID:Addgene_73946 Transfected construct (H. sapiens) mCherry-GABARAP This paper Subcloned from RRID:Addgene_73948 Transfected construct (H. sapiens) mCherry-GEC1 (GABARAPL1) This paper Subcloned from RRID:Addgene_73945 Transfected construct (H. sapiens) mCherry-GATE16 (GABARAPL2) This paper Subcloned from RRID:Addgene_73518 Transfected construct (H. sapiens) SNAP-WIPI2B WT This paper Subcloned from GFP-WIPI2B, PMID: 24954904 Transfected construct (H. sapiens) Halo-WIPI2B WT This paper Subcloned from GFP-WIPI2B, PMID: 24954904 Transfected construct (H. sapiens) SNAP-WIPI2B (FTTG) This paper Generated via quick change from SNAP-WIPI2B WT Continued on next page Stavoe et al. eLife 2019;8:e44219.

    Techniques: